Samples were mailed back to the lab, where qPCR was used to quantify numbers of viral RNA copies in breath specimens. Patients provided multiple samples per day over the entire course of infection. Participants watched a video online to learn how to collect samples. They recruited patients testing for COVID-19 from Northwestern Medicine. Scientists shipped the device to patients’ homes, allowing them to self-collect samples from their own home, which were mailed back to the lab for analysis. Talking, singing or shouting would likely increase amounts of exhaled virus. They used this device to test for the presence of the virus over 10 minutes of natural, relaxed breathing, establishing a conservative baseline of exhaled virus amount. It is not yet known what an infectious dose of viral airborne particles is.įor the study, Northwestern scientists created and patented a portable, non-invasive, inexpensive device to collect breath samples from infected people, and used qPCR to measure SARS-CoV-2 in those samples. “For example, if we assume the infectious dose for COVID is 300 copies, then a person who is exhaling 1,000 viral copies per minute would exhale an infectious dose in 20 seconds (highly risky in an elevator), whereas a person who is exhaling two viral copies per minute would exhale an infectious dose in about two hours (probably safe in an elevator),” Lane said. The study findings could be used to calculate the amount of time it takes for an individual to exhale an infectious dose of SARS-CoV-2, Lane. With this new device, investigators detected viral RNA in 100% of specimens collected from COVID-positive patients who were within 10 days of symptom onset and in none of the specimens collected from COVID-19 negative patients - a very high rate of accuracy. “We developed this easy, cheap method and used it to collect over 300 breath samples from 44 patients over the course of their infections - multiple samples a day over multiple days,” Lane said. To answer these questions, Zelano said, we needed a method to directly measure viral RNA on breath, repeatedly in the same patient over the course of their infection. The dynamics of viral shedding on breath over the course of infection are poorly understood, despite the fact that this is how the virus spreads.”Įxhaled levels of the virus drop steeply by day eight, Northwestern Medicine investigators findĪlso unknown is the relationship between levels of viral RNA on breath and symptom severity, vaccination status, virus variant and patient age and sex. “However, SARS-CoV-2 is spread through breath, and virus on the breath may not match virus in the nose. “The vast majority of research on viral loads over the course of a COVID-19 infection has been based on nasal or oral swabs, which measure virus in the nose or throat,” said lead study author Gregory Lane, senior research project manager in Zelano’s lab. “During COVID-19 infection, at what point are you exhaling a lot of virus, and when do you stop breathing it out?” “An important question in understanding transmission of SARS-CoV-2 is how much virus a patient is exhaling into the environment over the course of their infection and for how long,” said senior author Christina Zelano, assistant professor of neurology at Northwestern University Feinberg School of Medicine. The amount of virus being exhaled while infected was the same no matter which variant a person was infected with - people infected with Alpha exhaled just as much as those infected with Omicron, the study reports. Vaccinated and unvaccinated patients exhale similar levels of virus over the course of infection, the research shows. Mild and moderately symptomatic patients with COVID still exhale large amounts of virus, though severely symptomatic cases exhale higher levels on average, the study reports. The study will be published in eLife and has been posted as a pre-print. Northwestern investigators tested breath samples - collected multiple times daily from 44 individuals - over the entire course of infection to determine when a person is most infectious. On day eight, exhaled levels of virus drop steeply, down to near the limit of detection - an average of two copies exhaled per minute. It is the first longitudinal, direct measure of the number of SARS-CoV-2 viral copies exhaled per minute over the course of the infection - from the first sign of symptoms until 20 days after. COVID patients exhale high numbers of virus during the first eight days after symptoms start, as high as 1,000 copies per minute, reports a new Northwestern Medicine study.
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